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As you are probably aware, western music uses the letter names A B
C D E F G to describe notes. If you look at the guitar though, you can
see that an octave (from open string
to string fret 12 for example) is actually
divided into 12 different notes each fret producing a different note.

If you play the open E string
then play the note at fret 1, the pitch rises. This rise in pitch is
called a semitone. If you continue this up the string, after 12 semitones
you reach the octave E at fret 12.
So on the guitar, moving your finger along a string by one fret (which
is the same as shortening the length of the string by one fret) raises
the pitch by a semitone. If you move your finger along by two frets,
the pitch is raised by two semitones. Two semitones is equal to one
tone.
In order to name these 12 notes we need to add to the seven letter
names A B C D E F G. This is done by using sharps (#) and flats
(b). The 12 semitones along your E string
are named like this:

A sharp raises the pitch of a note by a semitone. A flat lowers the
pitch by a semitone. Clearly F sharp for example, is the same note as
G flat. When a note has two names like this the notes are said to be
enharmonic.
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